High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells
نویسندگان
چکیده
منابع مشابه
High Yield Production of Influenza Virus in Madin Darby Canine Kidney (MDCK) Cells with Stable Knockdown of IRF7
Influenza is a serious public health problem that causes a contagious respiratory disease. Vaccination is the most effective strategy to reduce transmission and prevent influenza. In recent years, cell-based vaccines have been developed with continuous cell lines such as Madin-Darby canine kidney (MDCK) and Vero. However, wild-type influenza and egg-based vaccine seed viruses will not grow effi...
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This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...
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Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-cont...
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Vaccination is one of the most cost-effective ways to prevent infection. Influenza vaccines propagated in cultured cells are approved for use in humans, but their yields are often suboptimal. Here, we screened A/Puerto Rico/8/34 (PR8) virus mutant libraries to develop vaccine backbones (defined here as the six viral RNA segments not encoding haemagglutinin and neuraminidase) that support high y...
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Insect cells such as High Five cells used in the manufacture of biopharmaceuticals are best grown in wavemixed bioreactors (1). This is due to the continual blending of foam with the culture medium which results from the wave-induced mixing and permanent renewal of the medium surface. Even the addition of an antifoam agent is not required. Process conditions which ensure maximum High Five cell ...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2021
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-020-11050-8